Phospho-STK4/3 (Thr183/Thr180) Recombinant Antibody

$Ramos (H) cells were treated with or without Staurosporine (1\u03bcM) for 1h, Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-STK4\/3 monoclonal antibody, unconjugated (bsm-62840R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Ramos (H) cells were treated with or without Staurosporine (1\u03bcM) for 1h, Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-STK4\/3 monoclonal antibody, unconjugated (bsm-62840R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62840r-100ul
Product Name	Phospho-STK4/3 (Thr183/Thr180) Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human STK4 around the phosphorylation site of T183
Modification Site	Thr183/Thr180
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6789
Swiss Prot	Q13043, Q13188
Subcellular location	Cytoplasm, Nucleus
Synonyms	KRS1; KRS2; MST1; MST2; STK3; STK4; YSK3.
Background	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Application Dilution
WB	1:300-5000