Phospho-SHIP1 (Tyr1022) Recombinant Monoclonal Antibody

$Western blot analysis of (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate. Using Phospho-SHIP1 (Tyr1022) (bsm-62834R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate. Using Phospho-SHIP1 (Tyr1022) (bsm-62834R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-62834r-100ul
Product Name	Phospho-SHIP1 (Tyr1022) Recombinant Monoclonal Antibody
Applications	WB, IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human INPP5D around the phosphorylation site of Y1022
Modification Site	Tyr1022
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3635
Swiss Prot	Q92835
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SHIP-1; p150Ship; hp51CN; INPP5D; SHIP.
Background	Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IP	1-2ug