UFC1 Recombinant Monoclonal Antibody

$Western blot analysis of A549 cell lysate. Using UFC1 (bsm-62735R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of A549 cell lysate. Using UFC1 (bsm-62735R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human

Overview
Catalog #	bsm-62735r-100ul
Product Name	UFC1 Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UFC1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51506
Swiss Prot	Q9Y3C8
Subcellular location	Extracellular, Secreted
Synonyms	HSPC155; Ubiquitin fold modifier conjugating enzyme 1; UFC1; Ufm1 conjugating enzyme 1.
Background	E1-like enzyme which specifically catalyzes the second step in ufmylation.Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF