PNMT Recombinant Monoclonal Antibody

$Western blot analysis of K562 cell lysate. Using PNMT (bsm-62565R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of K562 cell lysate. Using PNMT (bsm-62565R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-62565r-100ul
Product Name	PNMT Recombinant Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PNMTase
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5409
Swiss Prot	P11086
Subcellular location	Cytoplasm
Synonyms	PENT; PNMT; PNMTase.
Background	Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor. Other substrates include phenylethanolamine and octopamine. Also methylates normetanephrine.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200