Endo G Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Endo G monoclonal antibody, unconjugated (bsm-61741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Endo G monoclonal antibody, unconjugated (bsm-61741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61741r-100ul
Product Name	Endo G Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Endo G
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2021
Swiss Prot	Q14249
Subcellular location	Cytoplasm, Nucleus
Synonyms	EndoG; EndonucleaseG; Mitochondrial endonuclease G; NUCG_HUMAN.
Background	Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Application Dilution
WB	1:300-5000