CYP1A2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with CYP1A2 monoclonal antibody, unconjugated (bsm-61730R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CYP1A2 monoclonal antibody, unconjugated (bsm-61730R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-61730r-100ul
Product Name	CYP1A2 Recombinant Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cytochrome P450 1A2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1544
Swiss Prot	P05177
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CP12; CYP1A2; CYPIA2; P3 450; P450 4; P450 P3.
Background	A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200