SAE2 / UBA2 Recombinant Monoclonal Antibody

$Western blot analysis of A549 cell lysate. Using SAE2 \/ UBA2 (bsm-61707R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of A549 cell lysate. Using SAE2 \/ UBA2 (bsm-61707R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61707r-100ul
Product Name	SAE2 / UBA2 Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UBA2
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10054
Swiss Prot	Q9UBT2
Subcellular location	Cytoplasm, Nucleus
Synonyms	ARX; SAE2; UBA2; UBLE1B.
Background	The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF