KSR1 Recombinant Monoclonal Antibody

$Western blot analysis of HEK293 cell lysate. Using KSR1 (bsm-61694R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HEK293 cell lysate. Using KSR1 (bsm-61694R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-61694r-100ul
Product Name	KSR1 Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KSR1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8844
Swiss Prot	Q8IVT5
Subcellular location	Cytoplasm, Cell membrane
Synonyms	BKSR1; dKsr; EK31; KSR; KSR1; RSU2; SR31.
Background	Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases.Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF