RAP80 Recombinant Monoclonal Antibody

$Western blot analysis of 293T cell lysate. Using RAP80 (bsm-61657R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293T cell lysate. Using RAP80 (bsm-61657R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human

Overview
Catalog #	bsm-61657r-100ul
Product Name	RAP80 Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RAP80
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51720
Swiss Prot	Q96RL1
Synonyms	BRCA1-A complex subunit RAP80; RAP80; Receptor associated protein 80; RIP110; Rxrip110; UIMC1; X2HRIP110.
Background	Ubiquitin-binding protein.Specifically recognizes and binds 'Lys-63'-linked ubiquitin.Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF