EEF2 Recombinant Antibody

$Western blot analysis of (1) A431 cell lysate; (2) NIH\/3T3 cell lysate. Using EEF2 (bsm-61650R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) A431 cell lysate; (2) NIH\/3T3 cell lysate. Using EEF2 (bsm-61650R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61650r-100ul
Product Name	EEF2 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human eEF2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1938
Swiss Prot	P13639
Subcellular location	Cytoplasm
Synonyms	EEF 2; Eef2; EF-2; EF2; Elongation factor 2; Polypeptidyl tRNA translocase; SCA26.
Background	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF
Flow-Cyt	1ug/Test
ICC/IF	1:50-100