KDM1B Recombinant Monoclonal Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate. Using KDM1B (bsm-61638R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate. Using KDM1B (bsm-61638R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61638r-100ul
Product Name	KDM1B Recombinant Monoclonal Antibody
Applications	WB, FCM, IF(ICC), IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KDM1B
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	221656
Swiss Prot	Q8NB78
Subcellular location	Nucleus
Synonyms	AOF1; KDM1B; LSD2; Lysine-specific histone demethylase 1B; Lysine-specific histone demethylase 2.
Background	Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IP	1-2ug