Phospho-Cdc6 (Ser54) Recombinant Monoclonal Antibody

$Western blot analysis of (1) Raji cell lysate; (2) Raji + FBS cell lysate. Using Phospho-Cdc6 (Ser54) (bsm-61502R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) Raji cell lysate; (2) Raji + FBS cell lysate. Using Phospho-Cdc6 (Ser54) (bsm-61502R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-61502r-100ul
Product Name	Phospho-Cdc6 (Ser54) Recombinant Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CDC6 around the phosphorylation site of S54
Modification Site	Ser54
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	990
Swiss Prot	Q99741
Subcellular location	Cytoplasm, Nucleus
Synonyms	CDC6; CDC18L; CDC6-related protein; Cdc18-related protein; HsCDC6; HsCDC18; p62(cdc6).
Background	Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200