CHD4 Recombinant Monoclonal Antibody

$Western blot analysis of 293T cell lysate. Using CHD4 (bsm-61493R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293T cell lysate. Using CHD4 (bsm-61493R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-61493r-100ul
Product Name	CHD4 Recombinant Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHD4
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1108
Swiss Prot	Q14839
Subcellular location	Cytoplasm, Nucleus
Synonyms	ATP dependent helicase CHD4; CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta.
Background	ATP-dependent helicase that binds and distorts nucleosomal DNA.Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666.Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200