WNT5A Recombinant Monoclonal Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) SK-OV-3 cell lysate. Using WNT5A (bsm-61491R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) SK-OV-3 cell lysate. Using WNT5A (bsm-61491R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-61491r-100ul
Product Name	WNT5A Recombinant Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Wnt5a
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7474
Swiss Prot	P41221
Subcellular location	Extracellular, Secreted
Synonyms	hWNT5A; Protein Wnt5a; Wingless type MMTV integration site family member 5A; WNT 5A protein; WNT 5A protein precursor; WNT5A.
Background	Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200