Clathrin heavy chain Recombinant Monoclonal Antibody

$Western blot analysis of Hela cell lysate. Using Clathrin heavy chain (bsm-61487R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Hela cell lysate. Using Clathrin heavy chain (bsm-61487R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61487r-100ul
Product Name	Clathrin heavy chain Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Clathrin heavy chain 1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1213
Swiss Prot	Q00610,P53675
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CHC17; Clathrin heavy chain; Clathrin heavy chain like 1; CLH17; CLH22; CLTC; CLTCL; CLTD.
Background	Clathrin heavy chain proteins are composed of several functional domains, including a carboxy-terminal region that permits interaction with other heavy chain proteins within a triskelion, and a globular amino-terminal region that associates with other vesicle proteins. Adaptor proteins, such as AP2, epsin and EPS15, are responsible for the recruitment of vesicle proteins to sites of pit formation and the assembly of the clathrin-coated vesicle.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF