GCLC Recombinant Antibody

$Western blot analysis of HepG2 cell lysate. Using GCLC (bsm-61478R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Western blot analysis of HepG2 cell lysate. Using GCLC (bsm-61478R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Rat

Overview
Catalog #	bsm-61478r-100ul
Product Name	GCLC Recombinant Antibody
Applications	WB
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GCLC
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2729
Swiss Prot	P48506
Subcellular location	Cytoplasm
Synonyms	GCS heavy chain; Gamma-ECS Gamma-glutamylcysteine synthetase; GLCL; GLCLC; GCL.
Background	Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
Application Dilution
WB	1:300-5000