RAC1/2/3 Recombinant Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate; (3) PC-12 cell lysate. Using RAC1\/2\/3 (bsm-61458R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate; (3) PC-12 cell lysate. Using RAC1\/2\/3 (bsm-61458R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61458r-100ul
Product Name	RAC1/2/3 Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Rac1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5879
Swiss Prot	P63000,P15153,P60763
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Cell migration-inducing gene 5 protein; EN-7; GX; p21-Rac1; p21-Rac2; p21-Rac3; RAC1; RAC2; RAC3; TC25.
Background	In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive.
Application Dilution
WB	1:300-5000