PABP2 Recombinant Monoclonal Antibody

$Western blot analysis of HeLa cell lysate. Using PABP2 (bsm-61457R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HeLa cell lysate. Using PABP2 (bsm-61457R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-61457r-100ul
Product Name	PABP2 Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PABP 2
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8106
Swiss Prot	Q86U42
Subcellular location	Cytoplasm, Nucleus
Synonyms	Nuclear poly(A)-binding protein 1; PAB2; PABII; PABP2; PABPII; Pabpn1; poly(A) binding protein nuclear 1.
Background	Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity).Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity).Increases the affinity of poly(A) polymerase for RNA.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF