PRMT1 Recombinant Monoclonal Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate; (3) PC-12 cell lysate. Using PRMT1 (bsm-61453R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate; (3) PC-12 cell lysate. Using PRMT1 (bsm-61453R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IP
IF
ChIP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61453r-100ul
Product Name	PRMT1 Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IP, IF, ChIP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PRMT1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3276
Swiss Prot	Q99873
Subcellular location	Cytoplasm, Nucleus
Synonyms	Histone-arginine N-methyltransferase PRMT1; ANM1; HCP1; IR1B4; HRMT1L2.
Background	Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1 and NPRL2.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF
ChIP