NHERF1 Recombinant Antibody

$Western blot analysis of (1) HepG2 cell lysate; (2) PC12 cell lysate. Using NHERF1 (bsm-61378R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HepG2 cell lysate; (2) PC12 cell lysate. Using NHERF1 (bsm-61378R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-61378r-100ul
Product Name	NHERF1 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NHERF 1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9368
Swiss Prot	O14745
Synonyms	SLC9A3R1; EBP50; NPHLOP2; NHERF; NHERF-1; NHERF1.
Background	Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF