PCGF4/BMI1 Recombinant Monoclonal Antibody

$Western blot analysis of (1) K562 cell lysate; (2) PC-12 cell lysate. Using PCGF4\/BMI1 (bsm-61372R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) K562 cell lysate; (2) PC-12 cell lysate. Using PCGF4\/BMI1 (bsm-61372R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-61372r-100ul
Product Name	PCGF4/BMI1 Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BMI1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	100532731
Swiss Prot	P35226
Subcellular location	Cytoplasm, Nucleus
Synonyms	BMI1 ; Polycomb complex protein BMI 1; RING finger protein 51; RNF51; PCGF4.
Background	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF