CDCA3 Recombinant Antibody

$Western blot analysis of MCF7 cell lysate. Using CDCA3 (bsm-61345R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of MCF7 cell lysate. Using CDCA3 (bsm-61345R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61345r-100ul
Product Name	CDCA3 Recombinant Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CDCA3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	83461
Swiss Prot	Q99618
Subcellular location	Cytoplasm
Synonyms	Cell division cycle-associated protein 3; Trigger of mitotic entry protein 1; TOME-1; Gene-rich cluster protein C8; CDCA3; C8; GRCC8; TOME1.
Background	F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200