Notch 4 Recombinant Antibody

$Western blot analysis of Mouse lung lysate. Using NOTCH4 (bsm-61337R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Mouse lung lysate. Using NOTCH4 (bsm-61337R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-61337r-100ul
Product Name	Notch 4 Recombinant Antibody
Applications	WB, FCM
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Notch 4
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4855
Swiss Prot	Q99466
Subcellular location	Nucleus, Cell membrane
Synonyms	NOTC4; hNotch4; INT3; Notch homolog 4.
Background	Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
Application Dilution
WB	1:300-5000
FCM	1:20-100