ATF5 Recombinant Monoclonal Antibody

$Western blot analysis of (1) Jurkat cell lysate; (2) 3T3 cell lysate; (3) C6 cell lysate. Using ATF5 (bsm-61324R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) Jurkat cell lysate; (2) 3T3 cell lysate; (3) C6 cell lysate. Using ATF5 (bsm-61324R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61324r-100ul
Product Name	ATF5 Recombinant Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATF5
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22809
Swiss Prot	Q9Y2D1
Subcellular location	Cytoplasm, Nucleus
Synonyms	Cyclic AMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx; ATF5; ATFX; NAP1; NRIF3 associated protein; ODA 10.
Background	Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate.Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF