ASC/TMS1 Recombinant Monoclonal Antibody

$Western blot analysis of U937 cell lysate. Using ASC\/TMS1 (bsm-61273R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of U937 cell lysate. Using ASC\/TMS1 (bsm-61273R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-61273r-100ul
Product Name	ASC/TMS1 Recombinant Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ASC
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	29108
Swiss Prot	Q9ULZ3
Subcellular location	Cytoplasm, Nucleus
Synonyms	PYCARD; ASC; CARD5; TMS; TMS-1; TMS1.
Background	Functions as a key mediator in apoptosis and inflammation.Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner.Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200