EEA1 Recombinant Monoclonal Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate. Using EEA1 (bsm-61222R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate. Using EEA1 (bsm-61222R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61222r-100ul
Product Name	EEA1 Recombinant Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Early endosome antigen 1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8411
Swiss Prot	Q15075
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Early endosome antigen 1; EEA1; MST105; ZFYVE2.
Background	Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200