PHB1 Recombinant Monoclonal Antibody

$Western blot analysis of Ramos cell lysate. Using PHB1 (bsm-61208R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Ramos cell lysate. Using PHB1 (bsm-61208R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61208r-100ul
Product Name	PHB1 Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PHB1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5245
Swiss Prot	P35232
Synonyms	PHB; PHB1; HEL 215; Epididymis secretory sperm binding protein Li 54e.
Background	PHB1 is 30kDa tumor suppressor protein involved in cell cycle control. PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation. In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF