ATG12 Recombinant Monoclonal Antibody

$Western blot analysis of 293 cell lysate. Using ATG12 (bsm-61199R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293 cell lysate. Using ATG12 (bsm-61199R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human

Overview
Catalog #	bsm-61199r-100ul
Product Name	ATG12 Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG12
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9140
Swiss Prot	O94817
Subcellular location	Cytoplasm
Synonyms	APG12-like; APG12L; ATG12; Autophagy 12; Autophagy-related protein 12; FBR93; HAPG12; Ubiquitin-like protein ATG12.
Background	Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF