Cleaved-Caspase 3 p17 Recombinant Antibody

$Jurkat (H) cells were treated with or without etoposide treated (25uM) for 5 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Cleaved-Caspase 3 p17 monoclonal antibody, unconjugated (bsm-61090R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Jurkat (H) cells were treated with or without etoposide treated (25uM) for 5 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Cleaved-Caspase 3 p17 monoclonal antibody, unconjugated (bsm-61090R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61090r-100ul
Product Name	Cleaved-Caspase 3 p17 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	836
Swiss Prot	P42574
Subcellular location	Cytoplasm
Synonyms	Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF