Acetyl Lysine Recombinant Antibody

$Lane 1: 5% Input\uff1aHuman MCF-7 cell lysates; Lane 2: Immunoprecipitation using rabbit monoclonal IgG homotypic control; Lane 3: Immunoprecipitation with bsm-60865R\uff0c probed with Acetyl Lysine monoclonal Antibody, Unconjugated (bsm-60865R) at 1:50 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 5% Input\uff1aHuman MCF-7 cell lysates; Lane 2: Immunoprecipitation using rabbit monoclonal IgG homotypic control; Lane 3: Immunoprecipitation with bsm-60865R\uff0c probed with Acetyl Lysine monoclonal Antibody, Unconjugated (bsm-60865R) at 1:50 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human HeLa cell lysates; Lane 2: Human NIH\/3T3 cell lysates; Lane 3: Human BRL cell lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Mouse Kidney tissue lysates probed with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human NIH\/3T3 cell lysates; Lane 3: Human BRL cell lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Mouse Kidney tissue lysates probed with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: PTM-105RM pre adorbed with 3 \u03bc M synthetic Kac peptides; Lane 2: PTM-105RM pre adorbed with 3 \u03bc M unmodified peptides probed with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: PTM-105RM pre adorbed with 3 \u03bc M synthetic Kac peptides; Lane 2: PTM-105RM pre adorbed with 3 \u03bc M unmodified peptides probed with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60865r-100ul
Product Name	Acetyl Lysine Recombinant Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	N/A
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	PBS, Glycerol, BSA
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	N/A
Swiss Prot	N/A
Synonyms	Acetylated Lysine; Acetyllysine; Ac-lysine; Ac lysine; Ac-K; Ac K; Ac-Lys; Ac Lys; acetyl-Lysine; acetyl Lys; Ac-Lysine;
Application Dilution
WB	1:300-5000
IHC-P	1:200-400