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Heme Oxygenase 1 Monoclonal Antibody

Tissue: mouse liver \r\n Section type: Formalin-fixed & Paraffinembedded \r\n section \r\n Retrieval method: High temperature and high \r\n pressure \r\n Retrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\n Primary Ab dilution: 1:2000 \r\n Primary Ab incubation condition: 1 hour at \r\n room temperature \r\n Secondary Ab:SP Kit(Rabbit) (sp-0023) \r\n Polymer HRP (Ready to use) \r\n Counter stain: Hematoxylin (Blue) \r\n Comment: Color brown is the positive signal \r\n for bsm-60751R
Tissue: mouse liver \r\n Section type: Formalin-fixed & Paraffinembedded \r\n section \r\n Retrieval method: High temperature and high \r\n pressure \r\n Retrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\n Primary Ab dilution: 1:2000 \r\n Primary Ab incubation condition: 1 hour at \r\n room temperature \r\n Secondary Ab:SP Kit(Rabbit) (sp-0023) \r\n Polymer HRP (Ready to use) \r\n Counter stain: Hematoxylin (Blue) \r\n Comment: Color brown is the positive signal \r\n for bsm-60751R
Tissue: rat liver \r\n Section type: Formalin-fixed & Paraffinembedded \r\n section \r\n Retrieval method: High temperature and high \r\n pressure \r\n Retrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\n Primary Ab dilution: 1:2000 \r\n Primary Ab incubation condition: 1 hour at \r\n room temperature \r\n Secondary Ab:SP Kit(Rabbit) (sp-0023) \r\n Polymer HRP (Ready to use) \r\n Counter stain: Hematoxylin (Blue) \r\n Comment: Color brown is the positive signal \r\n for bsm-60751R
Tissue: rat liver \r\n Section type: Formalin-fixed & Paraffinembedded \r\n section \r\n Retrieval method: High temperature and high \r\n pressure \r\n Retrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\n Primary Ab dilution: 1:2000 \r\n Primary Ab incubation condition: 1 hour at \r\n room temperature \r\n Secondary Ab:SP Kit(Rabbit) (sp-0023) \r\n Polymer HRP (Ready to use) \r\n Counter stain: Hematoxylin (Blue) \r\n Comment: Color brown is the positive signal \r\n for bsm-60751R
Tissue: Human spleen \r\n Section type: Formalin-fixed & Paraffinembedded \r\n section \r\n Retrieval method: High temperature and high \r\n pressure \r\n Retrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\n Primary Ab dilution: 1:1000 \r\n Primary Ab incubation condition: 1 hour at \r\n room temperature \r\n Secondary Ab:SP Kit(Rabbit) (sp-0023) \r\n Polymer HRP (Ready to use) \r\n Counter stain: Hematoxylin (Blue) \r\n Comment: Color brown is the positive signal \r\n for bsm-60751R
Tissue: Human spleen \r\n Section type: Formalin-fixed & Paraffinembedded \r\n section \r\n Retrieval method: High temperature and high \r\n pressure \r\n Retrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\n Primary Ab dilution: 1:1000 \r\n Primary Ab incubation condition: 1 hour at \r\n room temperature \r\n Secondary Ab:SP Kit(Rabbit) (sp-0023) \r\n Polymer HRP (Ready to use) \r\n Counter stain: Hematoxylin (Blue) \r\n Comment: Color brown is the positive signal \r\n for bsm-60751R
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human 293T cell lysates; Lane 8: Human HepG2 cell lysates probed with Heme Oxygenase 1 Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human 293T cell lysates; Lane 8: Human HepG2 cell lysates probed with Heme Oxygenase 1 Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • FCM
  • IHC-P
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60751R
Product Name Heme Oxygenase 1 Monoclonal Antibody
Applications WB, FCM, IHC-P, ICC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human Heme Oxygenase 1
Clonality Monoclonal
Clone # -
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3162
Swiss Prot P09601
Subcellular location Cytoplasm
Synonyms Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; HMOX1; HO 1; HO-1; HO; HO1; Hsp32; bK286B10; D8Wsu38e; HMOX1_HUMAN.
Application Dilution
WB =1:500-2000
FCM FCM=1:100
IHC-P IHC-P=1:100-500
ICC ICC=1:50

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