Chk1 Recombinant Antibody

$Lane 1: Human A431 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human U-2 OS cell lysates probed with Chk1 Monoclonal Antibody, Unconjugated (bsm-60743R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human A431 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human U-2 OS cell lysates probed with Chk1 Monoclonal Antibody, Unconjugated (bsm-60743R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-60743r-100ul
Product Name	Chk1 Recombinant Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Chk1 :
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1111
Swiss Prot	O14757
Subcellular location	Cytoplasm, Nucleus
Synonyms	Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; CHEK-1; CHEK1; Chk 1; CHK1 checkpoint homolog (S. pombe); EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae); C85740; CHK1_HUMAN.
Application Dilution
WB	1:300-5000