PTP1B Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF7, 2: HeLa, 3: HCT-116, 4: \r\nHepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 50 kDa\r\nObserved MW: 50 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF7, 2: HeLa, 3: HCT-116, 4: \r\nHepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 50 kDa\r\nObserved MW: 50 kDa

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-60701r-100ul
Product Name	PTP1B Recombinant Antibody
Applications	WB
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PTP1B
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5770
Swiss Prot	P18031
Subcellular location	Cytoplasm, Cell membrane
Synonyms	PTN1_HUMAN; Tyrosine-protein phosphatase non-receptor type 1; EC:3.1.3.48; PTPN1; Protein-tyrosine phosphatase 1B (PTP-1B);
Application Dilution
WB	1:300-5000