PRAME Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103\r\novernight Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Ramos, 2: K562, 3: HeLa, 4: A375 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 58 kDa Observed MW: 58 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103\r\novernight Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Ramos, 2: K562, 3: HeLa, 4: A375 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 58 kDa Observed MW: 58 kDa

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-60694r-100ul
Product Name	PRAME Recombinant Antibody
Applications	WB
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PRAME
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23532
Swiss Prot	P78395
Subcellular location	Nucleus, Cell membrane
Synonyms	MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3.
Application Dilution
WB	1:300-5000