phospho-SIRT1 (Ser27) Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1-2: HEK-293+CA (-) (+), 3-4: Jurkat+CA(-) (+) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 82 kDa Observed MW: 120 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1-2: HEK-293+CA (-) (+), 3-4: Jurkat+CA(-) (+) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 82 kDa Observed MW: 120 kDa

Applications

WB
IHC-P
IHC-F

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60654r-100ul
Product Name	phospho-SIRT1 (Ser27) Recombinant Antibody
Applications	WB, IHC-P, IHC-F
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-SIRT1 (Ser27)
Modification Site	Ser27
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23411
Swiss Prot	Q96EB6
Subcellular location	Cytoplasm, Nucleus
Synonyms	SIR2L1; NAD-dependent protein deacetylase sirtuin-1; EC:2.3.1.286; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); SirtT1 75 kDa fragment; SIR1_HUMAN;
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500