IRF3 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-GSK-3 Beta (Ser9)) Monoclonal Antibody, Unconjugated (b bsm-56211M) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-GSK-3 Beta (Ser9)) Monoclonal Antibody, Unconjugated (b bsm-56211M) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

Reactivity

Human

Overview
Catalog #	bsm-56211m-100ul
Product Name	IRF3 Monoclonal Antibody
Applications	IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant protein of human IRF3
Clonality	Monoclonal
Clone #	5G9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3661
Swiss Prot	Q14653
Subcellular location	Cytoplasm, Nucleus
Synonyms	Interferon regulatory factor 3; IRF-3; IRF 3; IRF3_HUMAN.
Background	This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Application Dilution
IHC	1:100-500