Alpha SNAP Monoclonal Antibody

$Siha cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Alpha SNAP) monoclonal Antibody, Unconjugated (bsm-54737R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Siha cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Alpha SNAP) monoclonal Antibody, Unconjugated (bsm-54737R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54737R
Product Name	Alpha SNAP Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within C-terminal Human Alpha SNAP.
Clonality	Monoclonal
Clone #	6H6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8775
Swiss Prot	P54920
Subcellular location	Cell membrane
Synonyms	Alpha soluble NSF attachment protein antibody, Alpha-soluble NSF attachment protein antibody, N ethylmaleimide sensitive factor attachment protein alpha antibody, N-ethylmaleimide-sensitive factor attachment protein alpha antibody, napA antibody, NSF attachment protein alpha antibody, SNAA_HUMAN antibody, SNAP alpha antibody, SNAP-alpha antibody, SNAPA antibody
Background	Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs (also designated synaptobrevins), including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200