BAG2 Monoclonal Antibody

$Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54655R, 1\/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54655R, 1\/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

Reactivity

Human

Overview
Catalog #	bsm-54655R
Product Name	BAG2 Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N-terminal Human BAG2.
Clonality	Monoclonal
Clone #	2F9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	9532
Swiss Prot	O95816
Subcellular location	Cytoplasm, Cytoskeleton
Synonyms	BAG 2 antibody, BAG family molecular chaperone regulator 2 antibody, BAG-2 antibody, BAG-family molecular chaperone regulator-2 antibody, Bag2 antibody, BAG2_HUMAN antibody, BCL 2 associated athanogene 2 antibody, Bcl-2-associated athanogene 2 antibody, BCL2 associated athanogene 2 antibody, BCL2-associated athanogene 2 antibody, dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody, dJ417I1.2 antibody, KIAA0576 antibody, MGC149462 antibody, OTTHUMP00000016668 antibody
Background	BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq].
Application Dilution
WB	1:300-5000
FCM	1:20-100