Cytokeratin 9 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 9 Monoclonal Antibody, Unconjugated (bsm-54606R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 9 Monoclonal Antibody, Unconjugated (bsm-54606R) at 1:200 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IP
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54606R
Product Name	Cytokeratin 9 Monoclonal Antibody
Applications	WB, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein
Clonality	Monoclonal
Clone #	3F1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3857
Swiss Prot	P35527
Subcellular location	Nucleus, Extracellular exosome, Membrane
Synonyms	CK 9 antibody, CK-9 antibody, Cytokeratin 9 antibody, Cytokeratin-9 antibody, Cytokeratin9 antibody, EPPK antibody, K1C9_HUMAN antibody, K9 antibody, Keratin 9 antibody, Keratin antibody, Keratin type I cytoskeletal 9 antibody, Keratin-9 antibody, Keratin9 antibody, KRT 9 antibody, Krt9 antibody, Spermatid Perinuclear Ring Manchette Protein K9 antibody, type I cytoskeletal 9 antibody
Background	This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IP	1-2ug
IHC