FosB Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FosB Monoclonal Antibody, Unconjugated (bsm-54574R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FosB Monoclonal Antibody, Unconjugated (bsm-54574R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FosB Monoclonal Antibody, Unconjugated (bsm-54574R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FosB Monoclonal Antibody, Unconjugated (bsm-54574R) at 1:50 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IF(ICC)
IP
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54574R
Product Name	FosB Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IP, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein
Clonality	Monoclonal
Clone #	1C11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2354
Swiss Prot	P53539
Subcellular location	Nucleus
Synonyms	Activator protein 1 antibody, AP 1 antibody, DKFZp686C0818 antibody, FBJ murine osteosarcoma viral oncogene homolog B antibody, fosB antibody, FOSB_HUMAN antibody, G0/G1 switch regulatory protein 3 antibody, G0S3 antibody, GOS 3 antibody, GOS B antibody, GOS3 antibody, GOSB antibody, MGC42291 antibody, Oncogene FOS B antibody, Oncogene FOSB antibody, Protein fos B antibody, Protein fosB antibody
Background	Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, Fos B, (fos-related antigen) Fra 1, and Fra 2. Also named as DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IP	1-2ug
IHC