Phospho-YAP1 (S127) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-YAP1 (S127) Monoclonal Antibody, Unconjugated (bsm-54573R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-YAP1 (S127) Monoclonal Antibody, Unconjugated (bsm-54573R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-YAP1 (S127) Monoclonal Antibody, Unconjugated (bsm-54573R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-YAP1 (S127) Monoclonal Antibody, Unconjugated (bsm-54573R) at 1:50 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54573R
Product Name	Phospho-YAP1 (S127) Monoclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human breast carcinoma tissue, human kidney tissue, mouse kidney tissue.
Modification Site	Ser127
Clonality	Monoclonal
Clone #	5B5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10413
Swiss Prot	P46937
Subcellular location	Cytoplasm, Nucleus
Synonyms	65 kDa Yes associated protein antibody, 65 kDa Yes-associated protein antibody, COB1 antibody, YAp 1 antibody, YAP 65 antibody, YAP antibody, YAP1 antibody, YAP1_HUMAN antibody, YAP2 antibody, YAP65 antibody, yes -associated protein delta antibody, Yes associated protein 1 65kDa antibody, Yes associated protein 1 antibody, Yes associated protein 2 antibody, yes associated protein beta antibody, YKI antibody, Yorkie homolog antibody
Background	This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq].
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC