Phospho-Stat5(Y694) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-Stat5(Y694) Monoclonal Antibody, Unconjugated (bsm-54554R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-Stat5(Y694) Monoclonal Antibody, Unconjugated (bsm-54554R) at 1:50 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54554R
Product Name	Phospho-Stat5(Y694) Monoclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein
Modification Site	Tyr694
Clonality	Monoclonal
Clone #	3H8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6776
Swiss Prot	P42229
Subcellular location	Cytoplasm, Nucleus
Synonyms	MGF antibody, Signal transducer and activator of transcription 5A antibody, Signal Transducer and Activator of Transcription 5B antibody, STA5A_HUMAN antibody, STAT 5A antibody, STAT 5B antibody, STAT5 antibody, STAT5A antibody, STAT5B antibody, Transcription factor STAT5A antibody, Transcription factor STAT5B antibody
Background	The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC