Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-JAK2(Y1007+Y1008)) Monoclonal Antibody, Unconjugated (bsm-54504R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-JAK2(Y1007+Y1008)) Monoclonal Antibody, Unconjugated (bsm-54504R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Applications

WB
IHC-P
IF(ICC)
IP
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54504R
Product Name	Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IP, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-Peptide corresponding to residues surrounding Tyr1007 and 1008 of human JAK2.
Modification Site	Tyr1007+Tyr1008
Clonality	Monoclonal
Clone #	3G6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3717
Swiss Prot	O60674
Subcellular location	Cytoplasm, Nucleus, Endomembrane system
Synonyms	JAK 2 antibody, JAK-2 antibody, JAK2 antibody, JAK2_HUMAN antibody, Janus Activating Kinase 2 antibody, Janus kinase 2 (a protein tyrosine kinase) antibody, Janus kinase 2 antibody, JTK 10 antibody, JTK10 antibody, kinase Jak2 antibody, OTTHUMP00000043260 antibody, THCYT3 antibody, Tyrosine protein kinase JAK2 antibody, Tyrosine-protein kinase JAK2 antibody
Background	This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IP	1-2ug
IHC