RNF40 (8D8) Monoclonal Antibody

$Lane 1: Mouse testis; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY-5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: HepG2 Cells; Lane 9: A549 Cells; Probed with RNF40 (8D8) Monoclonal Antibody (bsm-54467R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse testis; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY-5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: HepG2 Cells; Lane 9: A549 Cells; Probed with RNF40 (8D8) Monoclonal Antibody (bsm-54467R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with RNF40 (8D8) Monoclonal Antibody (bsm-54467R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with RNF40 (8D8) Monoclonal Antibody (bsm-54467R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Applications

WB
IHC-P
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54467R
Product Name	RNF40 (8D8) Monoclonal Antibody
Applications	WB, IHC-P, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human RNF40 aa 200-400.
Clonality	Monoclonal
Clone #	8D8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	9810
Swiss Prot	SwissProt:
Subcellular location	Nucleus
Synonyms	E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40; RING-type E3 ubiquitin transferase BRE1B; KIAA0661
Background	Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IP	1-2ug