NDUFS4 (2F3) Monoclonal Antibody

$Lane 1: Rat Heart; Probed with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Heart; Probed with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Uterus tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Rat Uterus tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at 1:100, 30 min at room temperature, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Flow cytometric analysis of 293T cells with NDUFS4 (2F3) Monoclonal Antibody (bsm-54460R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54460R
Product Name	NDUFS4 (2F3) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length recombinant protein.
Clonality	Monoclonal
Clone #	2F3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4724
Swiss Prot	O43181
Subcellular location	Mitochondrion
Synonyms	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial; Complex I-18 kDa; Complex I-AQDQ; NADH-ubiquinone oxidoreductase 18 kDa subunit; CI-18 kDa; CI-AQDQ; NADH:ubiquinone oxidoreductase subunit S4; NDUFS4
Background	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IP	1-2ug