SRP54 (1A4) Monoclonal Antibody

$Lane 1: HepG2 Cells; Lane 2: MCF-7 Cells; Probed with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 Cells; Lane 2: MCF-7 Cells; Probed with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Flow cytometric analysis of HepG2 cells with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54457R
Product Name	SRP54 (1A4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human SRP54 aa 1-200.
Clonality	Monoclonal
Clone #	1A4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6729
Swiss Prot	P61011
Subcellular location	Cytoplasm, Nucleus
Synonyms	Signal recognition particle 54 kDa protein; SRP54
Background	SRP54, a component of the signal recognition particle (SRP), recognizes the signal peptide of secretory proteins and interacts with the signal recognition particle receptor to target the ribosome and the associated nascent chain to the endoplasmic reticulum. By functional analysis showed that SRP54 binds to SRP RNA via the M-domain, but only in the presence of RNA-bound SRP19, and that it associates with the signal peptide of nascent polypeptide chains. RNA interaction requires the presence of a loop in the C-terminal M-domain. Signal peptide recognition most likely involves methionine-rich loops. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts the signal recognition particle receptor, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IP	1-2ug