Cystathionine beta-synthase (1C2) Monoclonal Antibody

$Lane 1: 293 Cells; Lane 2: 293 Cells; Lane 3: Human Liver; Probed with Cystathionine beta-synthase (1C2) Monoclonal Antibody (bsm-54448R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cells; Lane 2: 293 Cells; Lane 3: Human Liver; Probed with Cystathionine beta-synthase (1C2) Monoclonal Antibody (bsm-54448R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Cystathionine beta-synthase (1C2) Monoclonal Antibody (bsm-54448R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Cystathionine beta-synthase (1C2) Monoclonal Antibody (bsm-54448R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54448R
Product Name	Cystathionine beta-synthase (1C2) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human CBS aa 400-550.
Clonality	Monoclonal
Clone #	1C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	875
Swiss Prot	P35520
Subcellular location	Cytoplasm, Nucleus
Synonyms	Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; CBS; Cystathionine beta synthase
Background	Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200