Syntaxin 3 (2B1) Monoclonal Antibody

$Lane 1: SH-SY5Y Cells; Lane 2: Human Kidney; Probed with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SH-SY5Y Cells; Lane 2: Human Kidney; Probed with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Pancreas tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Pancreas tissue incubated with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of 293T cells with Syntaxin 3 (2B1) Monoclonal Antibody (bsm-54446R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54446R
Product Name	Syntaxin 3 (2B1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein corresponding to cytoplasmic region of human Syntaxin 3.
Clonality	Monoclonal
Clone #	2B1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6809
Swiss Prot	Q13277
Subcellular location	Cell membrane
Synonyms	Syntaxin-3; STX3; STX3A
Background	Syntaxin-3 is targeted to the apical membrane of epithelial cells where it forms clusters and is important in establishing and maintaining polarity necessary for protein trafficking involving vesicle fusion and exocytosis. It is involved in docking of synaptic vesicles at presynaptic active zones.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400