CTBP1 (3A12) Monoclonal Antibody

$Lane 1: PC-3M Cells; Probed with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-3M Cells; Probed with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(IHC-P)
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54424R
Product Name	CTBP1 (3A12) Monoclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IF(ICC), IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human CtBP1 aa 300-500.
Clonality	Monoclonal
Clone #	3A12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1487
Swiss Prot	Q13363
Subcellular location	Cytoplasm, Nucleus
Synonyms	C-terminal-binding protein 1; CTBP1; CTBP; BARS; HADDTS
Background	CTBP1 is a corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. CTBP1 also binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200
IP	1-2ug