COMT (3F4) Monoclonal Antibody

$Lane 1: MCF-7 Cells; Lane 2: Rat Testis; Probed with COMT (3F4) Monoclonal Antibody (bsm-54419R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Lane 2: Rat Testis; Probed with COMT (3F4) Monoclonal Antibody (bsm-54419R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with COMT (3F4) Monoclonal Antibody (bsm-54419R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with COMT (3F4) Monoclonal Antibody (bsm-54419R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54419R
Product Name	COMT (3F4) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein of C-terminal human COMT.
Clonality	Monoclonal
Clone #	3F4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1312
Swiss Prot	P21964
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Catechol O-methyltransferase; COMT
Background	Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400